OTU_Misess: An integrated tool for OTU assignment and Microbial Identification of Sequences from Environmental SampleS

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Table 1.Library_certainty

Name	Description
Library	clone library name
Total_number	effective sequence number in the library
OTU_uncertain_0	the number of the sequences blast to the sequences of the public database, OTU in all hits over cutoff value are same
OTU_uncertain_1	hits over cutoff value with two kinds of OTU
OTU_uncertain_2	hits over cutoff value with three kinds of OTU
OTU_uncertain_3	hits over cutoff value with four kinds of OTU
OTU_uncertain_4	hits over cutoff value with five kinds of OTU
Otu_uncertain_10	no hit over cutoff value

Table 2.Library_self_OTU

Name	Description
Library	clone library name
Self_OTU_ID	local sequence OTU identity established by OTU_Misess
Number	sequence number in the library with local sequence OUT identity

Table 3.Library_pub_OTU

Name	Description
Pub_OTU_ID/Library	matrix of the Pub_OTU identity and the clone library

Table 4.Library_pub_OTU_taxo

Table 5.Clone_ident

Name	Description
Library	clone library name
Seqname	clone sequence name
OTU_uncertain_value	uncertain value of Pub_OTU identity
Max_similarity	maximal overall identity to known sequence
Self_OTU_ID	local sequence OTU identity established by OTU_Misess
Pub_OTU_ID	Pub_OTU identity
Brief_classification	brief Taxon
Full_classification	fully Taxon
Seq_desc	sequence description in clone library

Table 6.Clone_BLAST

Name	Description
QueryName	sequence name of the query sequence
QueryLength	query sequence length
Subjectlength	length of the subject (public) sequence
Query_coverage	blast overall coverage rate of query sequence
Subject_coverage	blast overall coverage rate of subject sequence
Matched_NCBI_Id	NCBI accession number of subject sequence
Score	highest blast hsp score
EValue	lowest blast hsp E-value
Identity	hsp identity
Similarity	sequence similarity
QueryStrand	the Strand of the query sequence
SubjectStrand	the strand of the subject sequence
QueryStart	hsp start of the query sequence
QueryEnd	hsp end of the query sequence
SubjectStart	hsp start of the subject sequence
SubjectEnd	hsp end of the subject sequence
Pub_OTU_ID	Pub_OTU identity
Brief_classification	brief Taxon
Full_classification	fully Taxon

Table 7.Clone_T_RF

Name	Description
Seqname	clone sequence name
Primer_Ford	location of the forward primer in the sequence
Primer_Back	location of the reverse primer in the sequence
Self_OTU_ID	local sequence OTU identity established by OTU_Misess
Ori_seqlen	length of the sequence before remove the primer sequence
Real_seqlen	length of the sequence after remove the primer sequence
T-RF_F by HhaI	forward terminal restriction site by HhaI
T-RF_F by HinfI	forward terminal restriction site by HinfI
T-RF_F by HaeIII	forward terminal restriction site by HaeIII
T-RF_F by MseI	forward terminal restriction site by MseI
T-RF_F by NlaIV	forward terminal restriction site by NlaIV
T-RF_R by HhaI	backward terminal restriction site by HhaI
T-RF_R by HinfI	backward terminal restriction site by HinfI
T-RF_R by HaeIII	backward terminal restriction site by HaeIII
T-RF_R by MseI	backward terminal restriction site by MseI
T-RF_R by NlaIV	backward terminal restriction site by NlaIV backward

Table 8.Primer_Location

Name	Description
Seqname	clone sequence name
Primer_Ford_Tag	tag of the location of the forward primer
Primer_Back_Tag	tag of the location of the reverse primer
Ori_seqlen	length of the sequence before remove the primer sequence
Real_seqlen	length of the sequence after remove the primer sequence
Primer_Ford_Begin	begin site of the location of the forward primer
Primer_Ford_End	end site of the location of the forward primer
Primer_Back_Begin	begin site of the location of the reverse primer
Primer_Back_End	end site of the location of the reverse primer

Last updated: January 8, 2008