Install Directions

• System requirements




- Tested on all common forms of Linux

- perl 5.80 or later version, Spreadsheet module.

- NCBI blast, download from NCBI, ftp://ftp.ncbi.nih.gov/blast

- Phred, Phrap and cross_match, download from http://www.phrap.org/

-Some .pm files used in Perl can be found in OTU_Misess/bin/perl_pm

 



• Installing




#unpacked OTU_Misess in /usr/local/soft/
[root@soft]$tar zxvf OTU_Misess.tar.gz
[root@soft]$chmod -R 755 OTU_Misess
[root@soft]$export PATH=$PATH:/usr/local/soft/OTU_Misess/bin



• Testing




#In /usr/local/soft/OTU_Misess/test directory, use these commands to test
#the program requirement, Phred, Phrap and blast program
# need to be correctly set in parameters files
[root@test]$Chromat2FastaA.pl ../lib/Chromat2FastaA.parameter
[root@test]$Fasta2xls_N.pl ../lib/Fasta2xls_N.parameter
#or you can use this command to test
[root@test]$OTU_Misess.pl ../lib/ A N



• Utilizing




#if your project need to assemble reads please set parameters following the
#Chromat2FastaA.parameter file, or you should set Chromat2FastaNA.parameter.
#The Fasta2xls_N.parameter is used for running Fasta2xls_N.pl with BLASTN, and Fasta2xls_TX.parameter is used for running Fasta2xls_TX with TBLASTX, if you want to
#use OTU_Misess.pl Project, FastaFileName and Fasta2Lib must be identically
#set ,write 'PATH=$PATH:/usr/local/soft/OTU_Misess/bin' in /etc/profile so
#that all users can use this program
[user@test]$OTU_Misess.pl ../lib/ A N