Supplementary meterial for the manuscript

• Test.Result [xls] size:142KB

Result of NAtest (../OTU_Misess/test/notassem/S_BT1TP2) in OTU_Misess package.

• Gut_Bacteria.Result [xls] size:1.63M

16S rRNA gene sequences were downloaded from NCBI to give an example of the user interface. The input file is from a study that examined the influences of obesity and kinship on microbial community composition in the mouse gut (Ley et al., Proc Natl Acad Sci U S A 2005, 102: 11070-11075). The data was not in chromatogram but Fasta format, therefore, information of sequence quality was not available. However, this result may be an good performance evaluation of OTU_Misses.

• Fungi.Result [xls] size:1.91M

18S rRNA genes and ITS sequences were applied to show the ability of OTU_Misess. Original source of these sequences is a paper studied by O’Brien et al. (Appl Environ Microbiol 2005, 71:5544-5550).

• nirS.Result [xls] size:280KB

165 nirS sequences were downloaded from NCBI to test the availability of nirS database and TBLASTX edition of OTU_Misess. Original source of these sequences was a paper studied by Ruiz-Rueda et al.(Int Microbiol 2007,10:253-260)

Software Download

• OTU_Misess[Zip] size:43.8M



The compressed file OTU_Misess.tar.gz/OTU_Misess.zip contains the main scripts and the the test data for installing and testing this program.

• OTU_list [zip] size: 402M



The compressed file OTU_list.zip contains the scripts for generating database.otu.list from FASTA file. FASTA sequences should have both gi and NCBI accession number in title.

Essential Dataset for the software

• 16S [zip] size: 28.9M



1. http://rdp.cme.msu.edu/hierarchy/hb_intro.jsp
Fasta file was made from RDP Release 10.9
2.http://greengenes.lbl.gov/Download/Taxonomic_Outlines/
Taxon list from RDP_SeqDescByOTU_tax_outline.txt at 20-Mar-200

• 18S_ITS [zip] size: 37.1M



NCBI CoreNucletide
Sequences were downloaded from NCBI: http://www.ncbi.nlm.nih.gov/ at 10-Mar-2009.
Sequences without taxonomic information were filtered out.



• nirS [zip] size: 39.1KB



Database was also downloaded from NCBI at 10-Mar-2009 and sequences without taxonomic information were filtered out.